CD8a antibody

Antigen: CD8a

Clonality: Monoclonal (76-2-11)

Host: Mouse

Reactivity: Pig (Porcine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Catalog no.: ABIN135476

Additional Information

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 76-2-11

Description: Porcine CD8a, Mr 32-34 kDa Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or a CD8 heterodimer. It is found on most thymocytes, and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition. MAb 76-2-11 reacts with the CD8 chain.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells), Spectral Red (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

anti-CD8a antibody

Publications

Publications: Pescovitz, Lunney, Sachs: "Preparation and characterization of monoclonal antibodies reactive with porcine PBL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 1, pp. 368-75, 1984 (PubMed).