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CD3 (CD3E) antibody (Biotin)

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (CT-3)
Host
Alternatives

Mouse
Reactivity
Alternatives

Chicken, Bird (Avian)
Conjugate
Alternatives Biotin
Application
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)
1 reference available
Catalog no. ABIN135506
Quantity 0,5 mg
Price 335.00 $   Plus shipping costs $35.00
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anti-CD3 (CD3E) antibody (Biotin)

Additional Information
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Alternative name CD3
Isotype IgG1
Clone CT-3
Description Chicken CD3 CD3 is a member of the T cell receptor-associated CD3 complex. MAb CT-3 recognizes a complex of at least three polypeptides of Mr 20-, 19-, and 17-kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90-kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50- and 40-kDa.

Application Details
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Application Notes Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.5 ug/10^6 cells), Spectral RedTM (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
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Publications Cheng, Zhu, Sun: "The immune enhancement of sodium lauryl sulfoacetate in chickens." in: Veterinary medicine international, Vol. 2010, pp. 485060, 2010 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (309), Rat (55), Rabbit (51), Hamster (38)
Reactivities Human (293), Mouse (Murine) (105), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (17), Cow (Bovine) (17), Monkey (15), Primate (9), Cat (Feline) (4), Bird (Avian) (3), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (353), Immunohistochemistry (Frozen Sections) (IHC (fro)) (92), Immunoprecipitation (IP) (65), Functional Studies (Func) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Western Blotting (WB) (42), Immunofluorescence (IF) (32), ELISA (19), Immunohistochemistry (IHC) (17), Blocking Antibody (Inhibition) (9), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (6), Cytotoxicity Test (CyTox) (2), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (45), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)