TCRgd antibody (Biotin)

Antigen: TCRgd

Clonality: Monoclonal (TCR-1)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Catalog no.: ABIN135518

Additional Information

Isotype: IgG1

Clone: TCR-1

Description: Chicken TCR MAb TCR1 precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates. Unlike human and mouse, in which cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of T cells.1-3 The frequency of TCR cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCR+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCR+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.

Application Details

Application Notes: WORKING DILUTIONS Flow Cytometry:Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Dalgaard, Norup, Pedersen et al.: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, 2010 (PubMed).