I-A antibody

Antigen: I-A

Clonality: Monoclonal (CIa)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Catalog no.: ABIN135545

Additional Information

Alternative name: Ia

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: CIa

Description: A 65 kDa glycoprotein consisting of a 32 kDa -chain non-covalently bound to a 27 kDa -chain The chicken Ia antigen is expressed on B cells, a subpopulation of the monocyte/macrophage lineage of cells, and mitogen-activated T cells. 1 The molecule recognized by this antibody is similar in structure to the murine Ia antigens and human DR antigens.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 /g106 cells), R-phycoerythrin (). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Ewert, Munchus, Chen et al.: "Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal antibody to monomorphic determinants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 5, pp. 2524-30, 1984 (PubMed).