Mouse anti-Human Immunoglobulin G (IgG) Antibody

Antigen: Immunoglobulin G (IgG)

Clonality: Monoclonal (JDC-10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN135618

Additional Information

Alternative name: IgG (Fc)

Format: Purified

Isotype: IgG1

Clone: JDC-10

Description: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA, may also react with IgG from other species.

Application Details

Application Notes: WORKING DILUTIONS Immunofluorescence: Purified (1 ug/10^6 cells), BIOT (1 ug/10^6 cells), R-PE (0.2 ug/10^6 cells). ELISA: AP (1:500-1:1,000), HRP (1:1,000-1:2,000), BIOT (1:1,000-1:2,000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8C or long-term at -20C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8C or long-term at -20C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Secondary Antibodies

Restrictions: For Research Use only