Mouse anti-Human IgE (Fc) Antibody (AP)

Antigen: IgE

Epitope: Fc

Clonality: Monoclonal (HP6029)

Host: Mouse

Reactivity: Human

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN135674

Additional Information

Alternative name: IgE (Fc)

Isotype: IgG2a

Clone: HP6029

Description: Reacts with the Fc portion of the heavy chain of human IgE as demonstrated by ELISA

Application Details

Application Notes: WORKING DILUTIONS ELISA: AP (1:500-1:1,000), HRP (1:1,000-1:2,000), BIOT (1:1,000-1:2,000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8C or long-term at -20C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8C or long-term at -20C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only