CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Catalog no.: ABIN135720

Additional Information

Alternative name: CD3

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: UCHT1

Description: Chain of the CD3/T cell antigen receptor complex CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa chain of the CD3/TCR complex.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), Biotin (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells), Allophycocyanin and Allophycocyanin-Cyanine 7 (10 L/10^6 cells),s Spectral RedTM (10 L/10^6 cells), Cyanine 5 (10 L/10^6 cells), R-phycoerythrin-Cyanine 5.5 (10 L/10^6 cells), R-phycoerythrin-Cyanine 7 (10 L/10^6 cells),

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) and Cyanine 5 (CYTM5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CYTM7), R-phycoerythrin-Cyanine 5.5 (R-PE-CYTM5.5), and allophycocyanin-Cyanine 7 (APC-CYTM7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only