ZDHHC12 antibody (AA 165-267)

Catalog No. ABIN1386098

The Rabbit Polyclonal anti-ZDHHC12 antibody has been validated for ELISA, WB, ICC, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect ZDHHC12 in samples from Human.

Quick Overview for ZDHHC12 antibody (AA 165-267) (ABIN1386098)

Target: ZDHHC12 (Zinc Finger, DHHC-Type Containing 12 (ZDHHC12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZDHHC12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZDHHC12 Antibody

Binding Specificity: AA 165-267

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZDHHC-12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ZDHHC12

Target: ZDHHC12 (Zinc Finger, DHHC-Type Containing 12 (ZDHHC12))

Alternative Name: ZDHHC-12

Background:

Synonyms: DHHC12, DHHC-12, Palmitoyltransferase ZDHHC12, ZDH12_HUMAN, Zdhhc12, Zinc finger DHHC domain-containing protein 12.

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZDHHC12 (zinc finger, DHHC domain containing 12), also known as ZNF400, is a 267 amino acid multi-pass membrane protein that contains one DHHC-type zinc finger and is thought to function as a palmitoyltransferase, catalyzing the transformation of palmitoyl-CoA and a cysteine-conjugated protein to a S-palmitoyl protein and free CoA. Three isoforms exist due to alternative splicing events. The gene encoding ZDHHC12 maps to human chromosome 9, which consists of about 145 million bases and 4 % of the human genome and encodes nearly 900 genes. Chromosome 9 is considered to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype.