SLC5A2 antibody (N-Term)

Catalog No. ABIN155150

The Rabbit Polyclonal anti-SLC5A2 antibody is suitable to detect SLC5A2 in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for SLC5A2 antibody (N-Term) (ABIN155150)

Target: SLC5A2 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 2 (SLC5A2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC5A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC5A2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects 74 kDa SGLT-2. A ~50-60 kDa band can also be detected, presumably represents the SGLT-2 cleavage fragments.

Purification: Affinity Chromatography on Protein A .

Immunogen: Synthetic peptide near the N-terminus of Mouse SGLT-2

Application Details

Application Notes: Western blotting (0.5-4 μg/mL). Jurkat cell lysate, mouse small intestine tissue lysate and rat kidney can be used asPositive Controls.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered saline (PBS) containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated freeze/defrost cycles.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. For long term storage aliquot and store at -70 °C.

Target Details for SLC5A2

Target: SLC5A2 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 2 (SLC5A2))

Alternative Name: SGLT2 / SLC5A2

Background: Sodium glucose co transporters (SGLTs) are located in the brush-border membrane, but transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUT). At least three members of SGLTs (SGLT 1-3) have been cloned and characterized. SGLTs transport a methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT-1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.Synonyms: Low affinity sodium-glucose cotransporter, Na(+)/glucose cotransporter 2, Sodium/glucose cotransporter 2, Solute carrier family 5 member 2

Gene ID: 6524

NCBI Accession: NP_003032

UniProt: P31639