D1-Int (PsbA) antibody

Antigen: D1-Int (PsbA)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis, Tobacco (Nicotiana tabacum), Cyanobacteria

Application: Western Blotting (WB)

Catalog no.: ABIN165360

Additional Information

Immunogen: synthetic peptide (a. a. 57 – 85) against PsbA2/3 proteins from Synechocystis sp. PCC 6803. The peptide is conservative in D1 protein sequences form all photosynthetic organisms.

Isotype: IgG  (Matching secondary antibodies)

Description: D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).

Specificity: Higher plants, cyanobacteria. Antibody reacts with a 32 kDa protein in all tested photosynthetic organisms

Application Details

Application Notes: Western blot (1 : 1.000 for ECL)

Storage: short term +4°C, long term -20°. Repeated freezing and thawing is not recommended.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Western blot analysis of Arabidopsis (At), tobacco (To) chloroplast and Synechocystis (Syn) thylakoid proteins with anti- PsbA-int

Publications

Publications: Takahashi, Whitney, Badger: "Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 9, pp. 3237-42, 2009 (PubMed).

Armbruster, Zühlke, Rengstl et al.: "The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly." in: The Plant cell, Vol. 22, Issue 10, pp. 3439-60, 2010 (PubMed). Further details: To quantify unambiguously the effect of the pam68-2 mutation on the rate of D1 synthesis, immunoprecipitation of the translation products employing a D1-specific antibody was performed

del Hoyo, Alvarez, del Campo et al.: "Oxidative stress induces distinct physiological responses in the two Trebouxia phycobionts of the lichen Ramalina farinacea." in: Annals of botany, Vol. 107, Issue 1, pp. 109-18, 2010 (PubMed). Method employed by authors: Western Blotting (WB)