D1-Nt (PsbA) antibody

Antigen: D1-Nt (PsbA)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis, Tobacco (Nicotiana tabacum), Cyanobacteria

Application: Western Blotting (WB)

Catalog no.: ABIN165361

Additional Information

Immunogen: synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.

Isotype: IgG  (Matching secondary antibodies)

Description: D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).

Specificity: Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.

Application Details

Application Notes: Western blot (1 : 2.000 for ECL)

Storage: short term +4°C, long term -20°. Repeated freezing and thawing is not recommended.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Western blot analysis of chloroplast (Arabidopsis and tobacco) and Synechocystis thylakoid proteins with anti-PsbA-Nt

Publications

Publications: Takahashi, Whitney, Badger: "Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 9, pp. 3237-42, 2009 (PubMed).

La: "Repression of both isoforms of disproportionating enzyme leads to higher malto-oligosaccharide content and reduced growth in potato." in: Planta, 2010 (PubMed).