CD74 antibody (AA 12-28)

Catalog No. ABIN1721492

Product Details anti-CD74 Antibody

Target: CD74 (HLA-DR-gamma (CD74))

Binding Specificity: AA 12-28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Reacts with human CD74.

Purification: Purified (protein G)

Immunogen: Synthetic peptide corresponding to the human CD74 invariant chain (aa 12-28) conjugated to KLH.

Clone: PIN1

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC (5 μg/mL) IP (12 μg/mL) WB (1 μg/mL) A doublet band of ~33-35 kDa is detected corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.

Positive control: PALA cell lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one year at -20°C. Avoid repeated freezing and thawing.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 (CD74 Products)

Background: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide "editing" in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).

HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD_antigen: CD74 Gene name : CD74

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints