SYT2 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742202

This anti-SYT2 antibody is a Rabbit Polyclonal antibody detecting SYT2 in WB, IP and IHC. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SYT2 antibody (Cytoplasmic Domain) (ABIN1742202)

Target: SYT2 (Synaptotagmin II (SYT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-SYT2 Antibody

Binding Specificity: AA 406-422, Cytoplasmic Domain

Specificity: Specific for synaptotagmin 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 406-422 in rat synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SYT2 antibody (ABIN1742202)

Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, (2012) (PubMed).

Culver, Savas, Park, Choi, Zheng, Zeitlin, Yates, Tanese: "Proteomic analysis of wild-type and mutant huntingtin-associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 26, pp. 21599-614, (2012) (PubMed).

Johnson, Franz, Kuhn, Furness, Rüttiger, Münkner, Rivolta, Seward, Herschman, Engel, Knipper, Marcotti: "Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses." in: Nature neuroscience, Vol. 13, Issue 1, pp. 45-52, (2009) (PubMed).

Target Details for SYT2

Target: SYT2 (Synaptotagmin II (SYT2))

Alternative Name: Synaptotagmin 2

Pathways: Synaptic Vesicle Exocytosis