SV2B antibody (AA 2-17)

Catalog No. ABIN1742257

This Mouse Monoclonal antibody specifically detects SV2B in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for SV2B antibody (AA 2-17) (ABIN1742257)

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SV2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 246E8

Product Details anti-SV2B Antibody

Binding Specificity: AA 2-17

Specificity: Specific for SV2 B.

Immunogen: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000 , ICC: 1:2000 up to 1:5000, IHC: 1:200 up to 1:500,

Comment:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

References for anti-SV2B antibody (ABIN1742257)

Aroeira, Vaz, Sebastião, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).

Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).

Target Details for SV2B

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Alternative Name: SV2 B