SCAMP1 antibody (AA 2-15)

Catalog No. ABIN1742263

This Rabbit Polyclonal antibody specifically detects SCAMP1 in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Rat, Mouse and Hamster and has been mentioned in 9+ publications.

Quick Overview for SCAMP1 antibody (AA 2-15) (ABIN1742263)

Target: SCAMP1 (Secretory Carrier Membrane Protein 1 (SCAMP1))

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SCAMP1 Antibody

Binding Specificity: AA 2-15

Specificity: Specific for SCAMP 1.

Purification: antiserum

Immunogen: Synthetic peptide SDFDSNPFADPDLN (aa 2-15 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IHC: 1 : 1000 up to 1 : 5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SCAMP1 antibody (ABIN1742263)

Zhao, Kim, Park, Park, Lee, Chang, Chang: "SCAMP5 plays a critical role in synaptic vesicle endocytosis during high neuronal activity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 30, pp. 10085-95, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Morciano, Beckhaus, Karas, Zimmermann, Volknandt: "The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels." in: Journal of neurochemistry, Vol. 108, Issue 3, pp. 662-75, (2009) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Antonin, Wagner, Riedel, Brose, Jahn: "Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion." in: Molecular and cellular biology, Vol. 22, Issue 5, pp. 1545-54, (2002) (PubMed).

Chamberlain, Burgoyne, Gould: "SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 10, pp. 5619-24, (2001) (PubMed).

Antonin, Holroyd, Tikkanen, Höning, Jahn: "The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3289-98, (2000) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for SCAMP1

Target: SCAMP1 (Secretory Carrier Membrane Protein 1 (SCAMP1))

Alternative Name: SCAMP 1