SYNPO antibody (Isoform 2)

Catalog No. ABIN1742348

The Rabbit Polyclonal anti-SYNPO antibody has been validated for WB, IHC and ICC. It is suitable to detect SYNPO in samples from Human, Mouse and Rat. There are 10+ publications available.

Quick Overview for SYNPO antibody (Isoform 2) (ABIN1742348)

Target: SYNPO (Synaptopodin (SYNPO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SYNPO Antibody

Binding Specificity: AA 331-452, Isoform 2

Specificity: Fragment used for immunization is present in all 3 isoforms.

Purification: antiserum

Immunogen: Recombinant mouse synaptopodin (aa 331-452 in isoform 2).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 600
IHC: 1 : 250 up to 1 : 500
IHC-P: 1 : 250 up to 1 : 500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SYNPO antibody (ABIN1742348)

Sugar, Wassenhove-McCarthy, Orr, Green, van Kuppevelt, McCarthy: "N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions." in: American journal of physiology. Renal physiology, Vol. 310, Issue 10, pp. F1123-35, (2016) (PubMed).

Fester, Zhou, Ossig, Labitzke, Bläute, Bader, Vollmer, Jarry, Rune: "Synaptopodin is regulated by aromatase activity." in: Journal of neurochemistry, Vol. 140, Issue 1, pp. 126-139, (2016) (PubMed).

Collier, OMalley, Rademacher, Stancati, Sisson, Sortwell, Paumier, Gebremedhin, Steece-Collier: "Interrogating the aged striatum: robust survival of grafted dopamine neurons in aging rats produces inferior behavioral recovery and evidence of impaired integration." in: Neurobiology of disease, Vol. 77, pp. 191-203, (2015) (PubMed).

Dieni, Nestel, Sibbe, Frotscher, Hellwig: "Distinct synaptic and neurochemical changes to the granule cell-CA3 projection in Bassoon mutant mice." in: Frontiers in synaptic neuroscience, Vol. 7, pp. 18, (2015) (PubMed).

Spada, Rojas, Pérez-Yagüe, Mulens, Cannata-Ortiz, Bragado, Barber: "NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery." in: Journal of leukocyte biology, Vol. 97, Issue 3, pp. 583-98, (2015) (PubMed).

Sun, Qu, Zhang, Caruana, Bertram, Li: "Glomerular endothelial cell injury and damage precedes that of podocytes in adriamycin-induced nephropathy." in: PLoS ONE, Vol. 8, Issue 1, pp. e55027, (2013) (PubMed).

Cohen, Louneva, Han, Hodes, Wilson, Bennett, Lucki, Arnold: "Chronic corticosterone exposure alters postsynaptic protein levels of PSD-95, NR1, and synaptopodin in the mouse brain." in: Synapse (New York, N.Y.), Vol. 65, Issue 8, pp. 763-70, (2011) (PubMed).

Chen, Wassenhove-McCarthy, Yamaguchi, Holzman, van Kuppevelt, Orr, Funk, Woods, McCarthy: "Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices." in: Kidney international, Vol. 78, Issue 11, pp. 1088-99, (2010) (PubMed).

Tarabra, Giunti, Barutta, Salvidio, Burt, Deferrari, Gambino, Vergola, Pinach, Perin, Camussi, Gruden: "Effect of the monocyte chemoattractant protein-1/CC chemokine receptor 2 system on nephrin expression in streptozotocin-treated mice and human cultured podocytes." in: Diabetes, Vol. 58, Issue 9, pp. 2109-18, (2009) (PubMed).

Vlachos, Korkotian, Schonfeld, Copanaki, Deller, Segal: "Synaptopodin regulates plasticity of dendritic spines in hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 4, pp. 1017-33, (2009) (PubMed).

Target Details for SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Alternative Name: Synaptopodin