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MHC Class I Polypeptide-Related Sequence A (MICA) antibody

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Synonyms Ica, mICA, FLJ60820, PERB11.1, MGC111087, MIC-A
(109), (14), (10), (9), (7), (7), (1)
(64), (42), (1)
Clonality (Clone)
Monoclonal ()
(8), (5), (5), (2), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(73), (46), (40), (28), (17), (13), (11), (11), (9), (4), (3), (2), (1), (1), (1)
Pubmed 6 references available
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Quantity 0.2 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused withcells of the P3 mouse myeloma cell line.
Clone 6D4
Isotype IgG2a
Specificity This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).
Cross-Reactivity (Details) Also reacts to: MICB
Characteristics Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
Purification Affinity chromatography on Protein G
Alternative Name MICA (MICA Antibody Abstract)
Background MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
Gene ID 4276
UniProt Q29983
Research Area Immunology, Innate Immunity, Adaptive Immunity
Application Notes Immunoprecipitation. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).

Jinushi, Takehara, Kanto et al.: "Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 3, pp. 1249-56, 2003 (PubMed).

Groh, Rhinehart, Randolph-Habecker et al.: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, 2001 (PubMed).

Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Groh, Rhinehart, Secrist et al.: "Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6879-84, 1999 (PubMed).

Groh, Steinle, Bauer et al.: "Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells." in: Science (New York, N.Y.), Vol. 279, Issue 5357, pp. 1737-40, 1998 (PubMed).

Catalog No. ABIN180912
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