MICA antibody (MHC Class I Polypeptide-Related Sequence A) (Biotin)

Details for Product anti-MICA Antibody No. ABIN94333
Antigen
Reactivity
Human, Non-Human Primate, Cow (Bovine), Cat (Feline)
142
20
14
14
14
6
4
1
Host
Mouse
87
53
Clonality (Clone)
Monoclonal ()
Conjugate
This MICA antibody is conjugated to Biotin
11
7
5
2
2
2
2
2
2
2
1
1
Application
ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
100
61
51
37
32
15
14
11
10
9
7
6
4
4
1
1
1
1
Options
Immunogen Membrane of human tonsil cells
Clone W6-32
Isotype IgG2a
Specificity The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types.
The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.
This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
No Cross-Reactivity Rabbit
Cross-Reactivity (Details) Human, Non-Human Primates, Bovine, Feline (Cat)
Antigen
Alternative Name HLA-Class I (MICA Antibody Abstract)
Background HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Pathways Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:300. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibody (Biotin) (ABIN94333) Surface staining of human peripheral blood cells with anti-HLA-class I(W6/32) Alexa F...
Product cited in: Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." in: Human immunology, Vol. 64, Issue 11, pp. 1039-44, 2003 (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, 2001 (PubMed).

Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." in: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, 1999 (PubMed).

Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." in: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, 1998 (PubMed).

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994 (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, 1986 (PubMed).

Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." in: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, 1982 (PubMed).

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, 1978 (PubMed).