CD57 antibody

Catalog No. ABIN181804

Product Details anti-CD57 Antibody

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Ion Exchange Chromatography.

Immunogen: Human neuroblastoma cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Clone: TB01

Isotype: IgM

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/10-1/100). Immunohistochemistry on Paraffin Sections (1/10-1/50): This product does not requireantigen retrieval using heat treatment prior to staining of paraffin sections but sodiumcitrate buffer may enhance staining. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Alternative Name: CD57 / HNK1 (B3GAT1 Products)

Background: CD57, also known as HNK-1, NK-1, and Leu-7, is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells and is also expressed on neural cells and striated muscle. CD57 is not expressed on red cells, granulocytes, monocytes, or platelets.While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).Synonyms: HNK-1, LEU7, NK-1, NK-Cell marker, NK1

Gene ID: 27087

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process