CLEC7A antibody

Catalog No. ABIN181895

The Rat Monoclonal anti-CLEC7A antibody has been validated for FACS, IP, IHC (fro) and Func. It is suitable to detect CLEC7A in samples from Mouse.

Quick Overview for CLEC7A antibody (ABIN181895)

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CLEC7A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 2A11

Product Details anti-CLEC7A Antibody

Specificity: This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3 % H202 should be performed after incubation with the primary antibody. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CLEC7A

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Alternative Name: CLEC7A / Dectin-1

Background: Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1

Gene ID: 56644

NCBI Accession: NP_064392

UniProt: Q6QLQ4

Pathways: Activation of Innate immune Response