WW Domain Containing Transcription Regulator 1 (WWTR1) antibody

Antigen: WW Domain Containing Transcription Regulator 1 (WWTR1)

Synonyms: WWTR1, TAZ

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Western Blotting (WB)

Catalog no.: ABIN182600

Additional Information

Alternative name: WWTR1 / TAZ

Gene ID: WWTR1

UniProt: Q9GZV5

Immunogen: The immunogen for anti-WWTR1 antibody: synthetic peptide directed towards the N terminal of human WWTR1

Sequence: PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQ QSYDVTDELP

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-WWTR1 antibody concentration is 1 mg/ml.

Description: TAZ (transctiptional coactivator with PDZ-binding motif), also known as WW domain containing transcription regulator 1 (WWTR1), is a member of the bicoid sub-family of homeodomain-containing transcription factors. TAZ is a 14-3-3 binding protein that co-activates Runx2-dependent gene transcription while repressing PPAR-gamma-dependent gene transcription. TAZ has been reported to be a modulator of mesenchymal stem cell differentiation. TAZ may also play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined.

Characteristics: Antigen Size: 400 amino acids

Molecular Weight: 44kDa

Application Details

Application Notes: WB Suggested Anti-WWTR1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-WWTR1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

Publications

Product: Hong, Hwang, McManus et al.: "TAZ, a transcriptional modulator of mesenchymal stem cell differentiation." in: Science (New York, N.Y.), Vol. 309, Issue 5737, pp. 1074-8, 2005 (PubMed).