ATP7A antibody (N-Term)

Catalog No. ABIN182880

The Rabbit Polyclonal anti-ATP7A antibody (ABIN182880) specifically detects ATP7A in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Horse, Sheep and Pig samples.

Quick Overview for ATP7A antibody (N-Term) (ABIN182880)

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP7A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP7A Antibody

Binding Specificity: N-Term

Sequence: MKKQIEAMGF PAFVKKQPKY LKLGAIDVER LKNTPVKSSE GSQQRSPSYQ

Specificity: The immunizing peptide used to raise this antibody is 100 % homologous to isoform 3 (503aa 54.3 kDa), 1 (1514aa, 165 kDa), 2 (1581aa, 172 kDa) and 5 (1422aa, 154 kDa) of human ATP7A.

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP7A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP7A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1500 AAAA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP7A

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Alternative Name: ATP7A

Background: The ATP7A gene encodes the Menkes copper-translocating P-type ATPase, a ubiquitous protein that regulates the absorption of copper in the gastrointestinal tract. Inside cells, this protein has a dual function: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper. The trafficking mechanism and catalytic activity combine to facilitate absorption and intercellular transport of copper. Menkes disease, a systemic copper deficiency disorder, is caused by mutations in the ATP7A gene.
Alias Symbols: MK, MNK, DSMAX, SMAX3
Protein Interaction Partner: ACIN1, UBC, COMMD1, CLU, ATOX1, PDZD11, CP, GLRX,
Protein Size: 1500aa

Molecular Weight: 163 kDa

Gene ID: 538

NCBI Accession: NM_000052, NP_000043

UniProt: Q04656

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process