MIER1 antibody (Middle Region)

Catalog No. ABIN183015

The Rabbit Polyclonal anti-MIER1 antibody (ABIN183015) specifically detects MIER1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Rabbit, Guinea Pig and Horse samples.

Quick Overview for MIER1 antibody (Middle Region) (ABIN183015)

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIER1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MIER1 Antibody

Binding Specificity: Middle Region

Sequence: RRTGDEKGVE AIPEGSHIKD NEQALYELVK CNFDTEEALR RLRF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MIER1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MIER1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 536 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MIER1

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Alternative Name: MIER1

Background: The association of hMI-ER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMI-ER1 proteins and this may have important implications for hMI-ER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A-sensitive histone deacetylase 1 (HDAC1).
Alias Symbols: ER1, MI-ER1, hMI-ER1, RP5-944N15.1
Protein Interaction Partner: TMEM171, SOX2, HDAC2, HDAC1, EHMT2, CDYL, TUBB1, APP, ELAVL1, UBC, CREBBP, SP1,
Protein Size: 536

Molecular Weight: 49 kDa

Gene ID: 57708

NCBI Accession: NM_020948, NP_065999

UniProt: Q8NES5

Pathways: Chromatin Binding