GJB1 antibody (C-Term)

Catalog No. ABIN183370

This Rabbit Polyclonal antibody specifically detects GJB1 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Horse, Rabbit and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for GJB1 antibody (C-Term) (ABIN183370)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GJB1 Antibody

Binding Specificity: C-Term

Sequence: GFGHRLSPEY KQNEINKLLS EQDGSLKDIL RRSPGTGAGL AEKSDRCSAC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GJB1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GJB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 283 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GJB1 antibody (ABIN183370)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1

Background: This gene encodes for a Connexin 32 protein. A large Charcot-Marie-Tooth disease family has been identified with a novel mutation in the Cx32 P2 promoter region at position -526bp. Cx32 mutants that are associated with a CNS phenotype may have toxic effects in oligodendrocytes.
Alias Symbols: CMTX, CX32, CMTX1
Protein Interaction Partner: UBC, VKORC1, APP, CNST, OCLN, PRKACA, SRC, PRKCA, GJB2, CALM1, CAV1, JUP,
Protein Size: 283

Molecular Weight: 31 kDa

Gene ID: 2705

NCBI Accession: NM_000166, NP_000157

UniProt: P08034

Pathways: Cell-Cell Junction Organization