TCEB3 antibody (C-Term)

Catalog No. ABIN184283

The Rabbit Polyclonal anti-TCEB3 antibody is suitable to detect TCEB3 in samples from Rat, Dog and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for TCEB3 antibody (C-Term) (ABIN184283)

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Reactivity: Rat, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCEB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TCEB3 Antibody

Binding Specificity: C-Term

Sequence: AYDGPSTSSA HLAPVVSSTV SYDPRKPTVK KIAPMMAKTI KAFKNRFSRR

Cross-Reactivity: Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 91%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against TCEB3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TCEB3

Application Details

Application Notes: WB Suggested Anti-TCEB3 Antibody Titration: 7.5 µg/mL
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 772 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TCEB3

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Alternative Name: TCEB3

Background: Elongin A (TCEB3), which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits.This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
Alias Symbols: EloA, SIII, TCEB3A, SIII p110
Protein Interaction Partner: KRTAP10-3, CEP57L1, SPERT, FAM9B, KRT40, CEP70, GMCL1, HOMEZ, TRIM54, MID2, PLK4, JAKMIP2, PSMB9, POLR2L, POLR2K, POLR2J, POLR2I, POLR2H, POLR2G, POLR2F, POLR2E, POLR2D, POLR2C, POLR2B, POLR2A, MDFI, UBC, RAD54B, MED26, ECT2, BARD1, CDK12, TCEB2, TCEB1, B
Protein Size: 772

Molecular Weight: 87 kDa

Gene ID: 6924

NCBI Accession: NM_003198, NP_003189

UniProt: Q14241