POU3F4 antibody (C-Term)

Catalog No. ABIN184335

Product Details anti-POU3F4 Antibody

Target: POU3F4 (POU Domain, Class 3, Transcription Factor 4 (POU3F4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU3F4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ADSLQLEKEV VRVWFCNRRQ KEKRMTPPGD QQPHEVYSHT VKTDTSCHDL

Cross-Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%

Characteristics: This is a rabbit polyclonal antibody against POU3F4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human POU3F4

Application Details

Application Notes: WB Suggested Anti-POU3F4 Antibody Titration: 0.3-0.5 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 361 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-POU3F4 antibody (ABIN184335)

Xia, Kikuchi, Minowa, Katori, Oshima, Noda, Ikeda: "Late-onset hearing loss in a mouse model of DFN3 non-syndromic deafness: morphologic and immunohistochemical analyses." in: Hearing research, Vol. 166, Issue 1-2, pp. 150-8, (2002) (PubMed).

Target Details for POU3F4

Target: POU3F4 (POU Domain, Class 3, Transcription Factor 4 (POU3F4))

Alternative Name: POU3F4 (POU3F4 Products)

Background: POU3F4 (brain-specific homeobox/POU domain protein 4, brain-4, Brn-4, transcription factor 4) is a transcription factor with a POU domain.
Alias Symbols: BRN4, DFN3, OTF9, DFNX2, BRAIN-4
Protein Interaction Partner: HNRNPU, POU3F3, POU3F1, POU3F2, POU3F4,
Protein Size: 361

Molecular Weight: 39 kDa

Gene ID: 5456

NCBI Accession: NM_000307, NP_000298

UniProt: P49335

Pathways: Sensory Perception of Sound