B-Cell CLL/lymphoma 7A (BCL7A) antibody

Antigen: B-Cell CLL/lymphoma 7A (BCL7A)

Synonyms: BCL7, cb585, MGC92023, sb:cb585, zgc:92023, id:ibd1097, wu:fk02d01, AI448316, 4432415N06Rik, bcl7a, MGC89576, BCL7A

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184562

Additional Information

Alternative name: BCL7A

Gene ID: 605, 77045 (mouse)

Immunogen: C-MKLEASQQNSEEM

Format: Ig-fraction

Description: All names and symbols: BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7. Official Symbol: BCL7A. RefSeq number(s): NP_066273. Gene ontology terms: actin binding

Specificity: Human, Mouse

Application Details

Application Notes: Western blot: Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2µg/ml. Please note that BCL7A, according to NP_066273, has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. This may be the reason why we see an approx 35kDa band rather than the predicted 26kDa band.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184562 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Zani, Asou, Jadayel et al.: "Molecular cloning of complex chromosomal translocation t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon." in: Blood, Vol. 87, Issue 8, pp. 3124-34, 1996 (PubMed).