phosphoglycerate Mutase 1/2/4 (PGAM1/2/4) antibody

Antigen: phosphoglycerate Mutase 1/2/4 (PGAM1/2/4)

Synonyms: PGAMA, PGAM-B, Pgmut, PGAM1, MGC128234, DKFZp469I0932

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184567

Additional Information

Alternative name: PGAM1 / PGAM2 / PGAM4

Gene ID: 5223, 5224, 441531

Immunogen: C-KAMEAVAAQGKAKK

Format: Affinity-purified

Description: All names and symbols: PGAM1, PGAM2, phosphoglycerate mutase 1 (brain), PGAMA, RP11-452K12.8, OTTHUMP00000059414, Phosphoglycerate mutase A, nonmuscle form, phosphoglycerate mutase 2 (muscle), MGC88743, PGAMM, Phosphoglycerate mutase, muscle form, PGAM3, phosphoglycerate mutase family member 4, PGAM-B, dJ1000K24.1, phosphoglycerate mutase family 3, phosphoglycerate mutase family 4, phosphoglycerate mutase processed protein. Official Symbol: PGAM1 / PGAM2 / PGAM4. RefSeq number(s): NP_002620.1, NP_000281.2, NP_001025062.1. Gene ontology terms: bisphosphoglycerate mutase activity, phosphoglycerate mutase activity, glycolysis, metabolism, cytosol, hydrolase activity, isomerase activity, bisphosphoglycerate phosphatase activity

Specificity: Please note this antibody is expected to recognize the products of 3 highly similar genes. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 28kDa band observed in human liver and brain lysates (predicted MW of 29kDa according to NP_002620). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184567 staining (0.5µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Sakoda, Shanske, DiMauro et al.: "Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family." in: The Journal of biological chemistry, Vol. 263, Issue 32, pp. 16899-905, 1988 (PubMed).