Fem-1 Homolog C (C. Elegans) (FEM1C) antibody

Antigen: Fem-1 Homolog C (C. Elegans) (FEM1C)

Synonyms: FEM1A, KIAA1785, EUROIMAGE686608, EUROIMAGE783647, AI646567, 2610312A07Rik, 3632443A22Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184578

Additional Information

Alternative name: FEM1C

Gene ID: 56929, 240263 (mouse)

Immunogen: C-HIPEKLETFVSLHR

Format: Affinity-purified

Description: All names and symbols: FEM1C, FEM1A, KIAA1785, EUROIMAGE686608, EUROIMAGE783647, fem-1 homolog c (C.elegans). Official Symbol: FEM1C. RefSeq number(s): NP_064562.1

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 75kDa band observed in heart and kidney lysates from mouse and rat (calculated MW of 68.6kDa according to mouse NP_775599.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184578 (0.5µg/ml) staining of rat kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Chan, Tan, Zhang et al.: "F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 45, pp. 32461-8, 1999 (PubMed).