Baculoviral IAP Repeat-Containing 7 (BIRC7) antibody

Antigen: Baculoviral IAP Repeat-Containing 7 (BIRC7)

Synonyms: KIAP, LIVIN, MLIAP, RNF50, ML-IAP, TIAP2, RGD1562883, BIRC7, zgc:165605, XLX, xEIAP, EIAP/XLX, Livin, E130019N06

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184599

Additional Information

Alternative name: LIVIN / BIRC7

Gene ID: 79444

Immunogen: C-RAPVRSRVRT

Format: Affinity-purified

Description: All names and symbols: BIRC7, LIVIN, baculoviral IAP repeat-containing 7 (livin), KIAP, MLIAP, mliap, ML-IAP, livin inhibitor-of-apotosis, kidney inhibitor of apoptosis protein, melanoma inhibitor of apoptosis protein, baculoviral IAP repeat-containing protein 7. Official Symbol: BIRC7. RefSeq number(s): NP_647478.1, NP_071444.1

Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: experiments gave bands at approx 30kDa and 35kDa in human acute lymphoblastic leukaemia MOLT4 lysate after 0.3 µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MW of 32.8kDa according to NP_647478.1and 30.9kDa according to NP_071444.1. Recommended concentration: 0.1 - 1.0 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184599 staining (0.3µg/ml) of MOLT4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kasof, Gomes: "Livin, a novel inhibitor of apoptosis protein family member." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3238-46, 2001 (PubMed).