B-Cell Linker (BLNK) antibody

Antigen: B-Cell Linker (BLNK)

Synonyms: Bca, BASH, Ly57, Ly-57, Lyw-57, SLP-65, LY57, SLP65, BLNK-S, MGC111051, BLNK, MGC112831, MGC127870, blnk, MGC147045

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184602

Additional Information

Alternative name: BLNK / SLP-65

Gene ID: 29760, 17060 (mouse)

Immunogen: C-KDSTRLKYAVKVS

Format: Affinity-purified

Description: All names and symbols: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa. Official Symbol: BLNK. RefSeq number(s): NP_037446.1, NP_001107566.1. Gene ontology terms: transmembrane receptor protein tyrosine kinase adaptor protein activity, hemocyte development, humoral immune response, intracellular signaling cascade, inflammatory response, cytoplasm

Specificity: This antibody is expected to recognize both reported isoforms (NP_037446.1, NP_001107566.1). Human, Mouse, Rat, Dog., Cow, Pig 456 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to 037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50kDa band is observed. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184602 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing BLNK (RC202488) and probed with ABIN184602 (mock transfection in first lane), tested by Origene.

Publications

Publications: Fu, Turck, Kurosaki et al.: "BLNK: a central linker protein in B cell activation." in: Immunity, Vol. 9, Issue 1, pp. 93-103, 1998 (PubMed).