GRB2-Related Adaptor Protein 2 (GRAP2) antibody

Antigen: GRB2-Related Adaptor Protein 2 (GRAP2)

Synonyms: P38, GADS, GRID, GRPL, GrbX, Mona, GRB2L, GRBLG, Grf40, GRAP-2, Gads, GrpL, MGC125066, GRAP2, zgc:112091, grap2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184603

Additional Information

Alternative name: GRAP2 / GRID / Grf40

Gene ID: 9402, 17444 (mouse)

Immunogen: C-GLFPANYVAPMTR

Format: Affinity-purified

Description: All names and symbols: GRAP2, GRID, P38, GADS, GRPL, GrbX, Mona, GRB2L, GRBLG, GRAP-2, GRB2-related adaptor protein 2, GRB-2-like protein, SH3-SH2-SH3 adaptor molecule, growth factor receptor-binding protein, GRB2-related protein with insert domain, hematopoietic cell-associated adaptor protein GRPL, growth factor receptor-bound protein 2-related adaptor protein 2. Official Symbol: GRAP2. RefSeq number(s): NP_004801.1. Gene ontology terms: SH3/SH2 adaptor protein activity, RAS protein signal transduction, intracellular signaling cascade, cell-cell signaling, antimicrobial humoral response (sensu Invertebrata), cytoplasm

Specificity: Human, Mouse 330 aa

Application Details

Application Notes: Western blot: Approx 38kDa band seen in Jurkat lysate. Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184603 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Qiu, Hua, Agrawal et al.: "Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1." in: Biochemical and biophysical research communications, Vol. 253, Issue 2, pp. 443-7, 1999 (PubMed).