CAPZB antibody (C-Term)

Catalog No. ABIN184616

There is 1 publication for this product available. The Goat Polyclonal anti-CAPZB antibody is suitable to detect CAPZB in samples from Human, Mouse, Rat and Pig. It has been validated for WB and ELISA.

Quick Overview for CAPZB antibody (C-Term) (ABIN184616)

Target: CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CAPZB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CAPZB Antibody

Binding Specificity: C-Term

Purpose: CAPZB

Sequence: NDLVEALKRK QQC

Specificity: This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence NDLVEALKRKQQC, from the C Terminus of the protein sequence according to NP_004921.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in lysates of cell lines Jurkat, HeLa and NIH-3T3 and approx 28 kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 30.6 kDa according to Human NP_004921.1 and 31.3 kDa according to Mouse NP_00103

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CAPZB antibody (ABIN184616)

Wang, Chen, Wu, Tsai, Shyue, Yang, Tzeng: "Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury." in: Journal of biomedical science, Vol. 18, pp. 13, (2011) (PubMed).

Target Details for CAPZB

Target: CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))

Alternative Name: CAPZB

Background: CAPZB, capping protein (actin filament) muscle Z-line, beta, CAPB, CAPZ, CAPPB, Cap Z, RP4-657E11.7, MGC104401, MGC129749, MGC129750, Cap Z, F-actin capping protein beta subunit

Gene ID: 832, 12345

NCBI Accession: NP_004921

Pathways: Regulation of Actin Filament Polymerization