Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB) antibody

Antigen: Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB)

Synonyms: zgc:66149, wu:fk65f06, CAPZB, MGC52754, DKFZp459M1515, CAPB, CAPZ, CAPPB, MGC104401, MGC129749, MGC129750, capzb

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184616

Additional Information

Alternative name: CAPZB

Gene ID: 832, 12345 (mouse)

Immunogen: NDLVEALKRKQQC

Format: Affinity-purified

Description: All names and symbols: CAPZB, capping protein (actin filament) muscle Z-line, beta, CAPB, CAPZ, CAPPB, Cap Z. Official Symbol: CAPZB. RefSeq number(s): NP_004921. Gene ontology terms: actin binding activity, actin capping protein complex, actin cytoskeleton

Specificity: This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form. Human, Mouse, Rat, Chicken, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 30kDa band observed in Hela, Jurkat and Placenta lysate (predicted MW of 31kDa according to NP_004921). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Images

ABIN184616 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hart, Cooper: "Vertebrate isoforms of actin capping protein beta have distinct functions In vivo." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1287-98, 2000 (PubMed).

Wang, Chen, Wu et al.: "Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury." in: Journal of biomedical science, Vol. 18, pp. 13, 2011 (PubMed).