PML-RARA Regulated Adaptor Molecule 1 (PRAM1) antibody

Antigen: PML-RARA Regulated Adaptor Molecule 1 (PRAM1)

Synonyms: PRAM-1, PML-RAR, MGC39864, RGD1564330, AY665714, PRAM1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184622

Additional Information

Alternative name: PRAM1

Gene ID: 84106

Immunogen: DFCDPLENQPLPLGR

Format: Affinity-purified

Description: All names and symbols: PRAM-1, PRAM-1 protein, PML-RARA target gene encoding an Adaptor Molecule-1. Official Symbol: PRAM1. RefSeq number(s): NP_115528

Specificity: Human

Application Details

Application Notes: Western blot: Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3µg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001, 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184622 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Moog-Lutz, Peterson, Lutz et al.: "PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22375-81, 2001 (PubMed).