Dual Specificity Phosphatase 1 (DUSP1) antibody

Antigen: Dual Specificity Phosphatase 1 (DUSP1)

Synonyms: HVH1, MKP1, CL100, MKP-1, PTPN10, Mkp1, 3CH134, Ptpn16, DUSP1, MGC128230, erp, mkp-1, U19515, cb269, sb:cb269, zgc:55920, id:ibd2586, wu:fd19g02, wu:fe50g09

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184638

Additional Information

Alternative name: Dual specificity protein phosphatase 1 (DUSP1/MKP-1)

Gene ID: 1843, 19252 (mouse)

Immunogen: Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1

Description: All names and symbols: DUSP1, dual specificity phosphatase 1, HVH1, CL100, MKP-1, PTPN10, serine/threonine specific protein phosphatase, MKP1. Official Symbol: DUSP1. RefSeq number(s): NP_004408.1. Gene ontology terms: protein phosphatase type 2C activity, protein phosphatase type 2A activity, non-membrane spanning protein tyrosine phosphatase activity, cell cycle, response to oxidative stress, protein amino acid dephosphorylation, calcium-dependent protein serine/threonine phosphatase activity, magnesium-dependent protein serine/threonine phosphatase activity, CTD phosphatase activity, hydrolase activity, MAP kinase phosphatase activity, myosin phosphatase activity, protein phosphatase type 2B activity.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 40kDa band observed in Human lysates of HeLa (calculated MW of 39.3kDa according to NP_004408.1). Recommended concentration: 0.3-1µg/ml. Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184638 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ABIN184638 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Alessi, Smythe, Keyse: "The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts." in: Oncogene, Vol. 8, Issue 7, pp. 2015-20, 1993 (PubMed).