Docking Protein 3 (DOK3) antibody

Antigen: Docking Protein 3 (DOK3)

Synonyms: DOKL, FLJ22570, FLJ39939, Dokl, AI450713, MGC151299, DOK3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184642

Additional Information

Alternative name: DOK3

Gene ID: 79930, 27261 (mouse)

Immunogen: TRERRKGPAPCDRP

Format: Affinity-purified

Description: All names and symbols: DOK3, Docking Protein 3, FLJ22570, FLJ39939. Official Symbol: DOK3. RefSeq number(s): NP_079148.1. Gene ontology terms: insulin receptor binding activity, protein binding activity, RAS protein signal transduction, cytoplasm

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 60-65kDa band observed in Human PBMC lysate (predicted MW of 57kDa according to NP_079148.1). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

ABIN184642 staining (1µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Lemay, Davidson, Latour et al.: "Dok-3, a novel adapter molecule involved in the negative regulation of immunoreceptor signaling." in: Molecular and cellular biology, Vol. 20, Issue 8, pp. 2743-54, 2000 (PubMed).