ATF4 antibody

Antigen: ATF4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184656

Additional Information

Gene ID: 468, 11911 (mouse), 79255 (rat)

Immunogen: C-EEVRKARGKKRVP

Format: Affinity-purified

Description: All names and symbols: ATF4, activating transcription factor 4 (tax-responsive enhancer element B67), CREB2, TXREB, CREB-2, TAXREB67. Official Symbol: ATF4. RefSeq number(s): NP_001666. Gene ontology terms: RNA polymerase II transcription factor activity, DNA binding, regulation of transcription, DNA-dependent, nucleus

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Western blot: Approx 40kDa band seen in Raji cell lysate. Recommended for use at 1-3 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184656 staining (1µg/ml) of Raji lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tsujimoto, Nyunoya, Morita et al.: "Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I." in: Journal of virology, Vol. 65, Issue 3, pp. 1420-6, 1991 (PubMed).