LIM and SH3 Protein 1 (LASP1) (N-Term) antibody

Antigen: LIM and SH3 Protein 1 (LASP1)

Synonyms: MLN50, Lasp-1, Def-4, SH3P6, AA408629, Tg(Col1a1-lacZ)1Ngma, fb92f05, zgc:77542, wu:fb92f05, LASP1, MGC128762, DKFZp459O1227, lasp1, MGC53336, MGC89667, mln50, lasp-1

Binding Site: N-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184658

Additional Information

Alternative name: LASP1

Gene ID: 3927, 16796 (mouse), 29278 (rat)

Immunogen: NPNCARCGKIVYP

Description: All names and symbols: LASP1, LIM and SH3 protein 1, MLN50, Lasp-1, LIM and SH3 protein. Official Symbol: LASP1. RefSeq number(s): NP_006139.1. Gene ontology terms: SH3/SH2 adaptor protein activity

Specificity: Human, Mouse, Rat, Pig, Chicken

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 35kDa band observed in Human Brain (Cerebellum) and Human Colon, Duodenum, Liver and Pancreas lysates (calculated MW of 29.7kDa according to NP_006139.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184658 staining (0.1µg/ml) of Human Brain (Cerebellum) extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Schreiber, Moog-Lutz, Regnier et al.: "Lasp-1, a novel type of actin-binding protein accumulating in cell membrane extensions." in: Molecular medicine (Cambridge, Mass.), Vol. 4, Issue 10, pp. 675-87, 1999 (PubMed).