Nitric Oxide Synthase 1 (Neuronal) (NOS1) antibody

Antigen: Nitric Oxide Synthase 1 (Neuronal) (NOS1)

Synonyms: NOS, nNOS, IHPS1, INOS, NOS2A, HEP-NOS, NO, bNOS, NOS-I, Nos-1, 2310005C01Rik, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, iNos, nos, NOS2, NOS1, nos2

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184662

Additional Information

Alternative name: NOS1

Gene ID: 4842

Immunogen: C-ESKKDTDEVFSS

Format: Affinity-purified

Description: All names and symbols: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS. Official Symbol: NOS1. RefSeq number(s): NP_000611.1. Gene ontology terms: nitric oxide synthase activity, calmodulin binding, electron transporter activity, nitric oxide biosynthesis, electron transport, synaptic transmission, muscle contraction, intracellular signaling cascade, cell-cell signaling, membrane fraction, oxidoreductase activity

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 160-170kDa band observed in Human Skeletal Muscle and Mouse Brain lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurotransmitters

Restrictions: For Research Use only

Images

ABIN184662 staining (0.3µg/ml) of human muscle extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Fujisawa, Ogura, Kurashima et al.: "Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines." in: Journal of neurochemistry, Vol. 63, Issue 1, pp. 140-5, 1994 (PubMed).