Protein Kinase C and Casein Kinase Substrate in Neurons 3 (PACSIN3) antibody

Antigen: Protein Kinase C and Casein Kinase Substrate in Neurons 3 (PACSIN3)

Synonyms: SDPIII, AW413130, 4921507A02Rik, 6330413E15Rik, SdpIII, Pacsin1, MGC56324, MGC82145, PACSIN3, sdpiii

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184666

Additional Information

Alternative name: PACSIN3

Gene ID: 29763

Immunogen: APEEDAGGEALGGS-C

Format: Affinity-purified

Description: All names and symbols: PACSIN3, protein kinase C and casein kinase substrate in neurons 3, SH3 domain-containing protein 6511, SDPIII, syndapin III. Official Symbol: PACSIN3. RefSeq number(s): NP_057307.2. Gene ontology terms: cytoplasm, cytoskeletal protein binding, endocytosis, enzyme binding, negative regulation of endocytosis

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in H460 and Human Heart and Skeletal Muscle lysates (predicted MW of 49kDa according to NP_057307.2). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184666 staining (1µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Cuajungco, Grimm, Oshima et al.: "PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18753-62, 2006 (PubMed).