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Details for Product No. ABIN184670

Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody

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Antigen
Synonyms ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, grb2, wu:fb18e12, wu:fb34e06, zgc:73077, GRB2, GRAP, grap, zgc:110202, MGC109892, si:dkeyp-9d4.1
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN184670
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name GRB2
Background Synonyms: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885, 14784, 81504
NCBI Accession NP_002077.1, NP_987102.1
Research Area Cancer
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in Jurkat and NIH3T3 lysates. Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration: 0.1-0.3 µg/mL. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained (please contact technical support for details).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Lowenstein, Daly, Batzer et al.: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992 (PubMed).

Alternatives for antigen "Growth Factor Receptor-Bound Protein 2 (GRB2)", type "Antibodies"
Hosts (60), (31), (12), (1)
Reactivities (101), (49), (48), (17), (12), (9), (2), (2), (1)
Applications (79), (36), (34), (13), (10), (10), (9), (5), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (6), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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