Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody

Details for Product No. ABIN184670
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Antigen
Synonyms GRB2, grb2, AA408164, Ash, ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, wu:fb18e12, wu:fb34e06, zgc:73077
Epitope
C-Term
(17), (10), (9), (8), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(98), (38), (37), (6), (4), (1), (1)
Host
Goat
(66), (24), (10), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(90), (43), (32), (23), (10), (7), (6), (5), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184670
374.27 $
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Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name GRB2
Background Synonyms: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885, 14784, 81504
NCBI Accession NP_987102, NP_002077
Research Area Cancer
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in Jurkat and NIH3T3 lysates. Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration: 0.1-0.3 µg/mL. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained (please contact technical support for details).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (2) ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
General Lowenstein, Daly, Batzer et al.: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992 (PubMed).

Validation Images
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