GRB2 antibody (AA 89-118)

Catalog No. ABIN3031114

Product Details anti-GRB2 Antibody

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Binding Specificity: AA 89-118

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Xenopus

Purification: Purified

Immunogen: A portion of amino acids 89-118 from the human protein was used as the immunogen for this GRB2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the GRB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the GRB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2 (GRB2 Products)

Synonyms: GRB2 antibody, grb2 antibody, AA408164 antibody, Ash antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, wu:fb18e12 antibody, wu:fb34e06 antibody, zgc:73077 antibody, growth factor receptor bound protein 2 antibody, growth factor receptor-bound protein 2 antibody, growth factor receptor-bound protein 2b antibody, GRB2 antibody, grb2 antibody, Grb2 antibody, grb2b antibody

Background: GRB2 is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. [UniProt]

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation