Forkhead Box P3 (FOXP3) antibody

Antigen: Forkhead Box P3 (FOXP3)

Synonyms: HNF3A, TCF3A, MGC33105, sf, JM2, scurfin, AIID, IPEX, PIDX, XPID, DIETER, MGC141961, MGC141963, RGD1562112, FOXP3, foxp3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN184677

Additional Information

Alternative name: FOXP3 / SCURFIN

Gene ID: 50943

Immunogen: SQRPSRCSNPTPGP

Format: Affinity-purified

Description: All names and symbols: FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked. Official Symbol: FOXP3. RefSeq number(s): NP_054728.2. Gene ontology terms: transcription factor activity, regulation of transcription, DNA-dependent, immune response, nucleus

Specificity: Human 441 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Immunology, Transcription Factors, Cell Cycle

Restrictions: For Research Use only

Images

ABIN184677 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.

Publications

Publications: Rao, Petrone, Ponath: "Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1446-55, 2005 (PubMed).

Miyara, Amoura, Parizot et al.: "The immune paradox of sarcoidosis and regulatory T cells." in: The Journal of experimental medicine, Vol. 203, Issue 2, pp. 359-70, 2006 (PubMed).