Forkhead Box P3 (FOXP3) antibody

Antigen: Forkhead Box P3 (FOXP3)

Synonyms: HNF3A, TCF3A, MGC33105, sf, JM2, scurfin, AIID, IPEX, PIDX, XPID, DIETER, MGC141961, MGC141963, RGD1562112, FOXP3, foxp3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Chromatin Immunoprecipitation (ChiP)

Catalog no.: ABIN184677

Additional Information

Alternative name: FOXP3 / SCURFIN

Gene ID: 50943

Swiss-Prot: NP_054728.2

Immunogen: Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

Cross-Reactivity: Human, Dog (Canine), Pig (Porcine)

Format: Liquid

Description: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1). Principal Names: scurfin, FOXP3delta7, MGC141963, MGC141961, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, JM2 protein, DIETER, XPID, PIDX, IPEX, AIID, JM2, forkhead box P3, SCURFIN, FOXP3. Official Symbol: FOXP3

Specificity: Human 441 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: This antibody has been successfully used in WB for human T regulatory cells as described in the following paper: Godfrey et al, Blood. 2005 Jan 15,105(2):750-8, PMID: 15374887. IHC: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 µg/ml. Chromatin immunoprecipitation: This antibody has been successfully used in ChIP from activated human T cells as described in the following paper: Stahl et al, PLoS One. 2009 Sep 24,4(9):e7158, PMID: 19777054.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Immunology, Transcription Factors, Cell Cycle

Restrictions: For Research Use only

Images

ABIN184677 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.

Publications

Publications: Rao, Petrone, Ponath: "Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1446-55, 2005 (PubMed).

Miyara, Amoura, Parizot et al.: "The immune paradox of sarcoidosis and regulatory T cells." in: The Journal of experimental medicine, Vol. 203, Issue 2, pp. 359-70, 2006 (PubMed).