Engulfment and Cell Motility 3 (ELMO3) antibody

Antigen: Engulfment and Cell Motility 3 (ELMO3)

Synonyms: CED12, CED-12, ELMO-3, FLJ13824, MGC124673, TMEM208, ELMO3, MGC166191

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184678

Additional Information

Alternative name: ELMO3

Gene ID: 79767

Immunogen: TNFNFCYDCSIAEP

Format: Affinity-purified

Description: All names and symbols: ELMO3, engulfment and cell motility 3 (ced-12 homolog, C. elegans), CED12, FLJ13824, ced-12 homolog 3, engulfment and cell motility 3. Official Symbol: ELMO3. RefSeq number(s): NP_078988.2

Specificity: Human

Application Details

Application Notes: Western blot: Approx 70kDa band seen in Human Brain lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

ABIN184678 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Gumienny, Brugnera, Tosello-Trampont et al.: "CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration." in: Cell, Vol. 107, Issue 1, pp. 27-41, 2001 (PubMed).