Transmembrane Protease, Serine 5 (TMPRSS5) antibody

Antigen: Transmembrane Protease, Serine 5 (TMPRSS5)

Synonyms: SPINESIN, MGC141886, MGC148044, Amp, TMPRSS5

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184703

Additional Information

Alternative name: Spinesin / TMPRSS5

Gene ID: 80975

Immunogen: C-LDWIHDTAQDSLL

Format: Affinity-purified

Description: All names and symbols: TMPRSS5, SPINESIN, transmembrane protease, serine 5 (spinesin), MGC141886, MGC148044. Official Symbol: TMPRSS5. RefSeq number(s): NP_110397.1. Gene ontology terms: trypsin activity, proteolysis and peptidolysis, integral to membrane, chymotrypsin activity, hydrolase activity

Specificity: Human, Mouse, Rat 457 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex) lysates and in Mouse and Rat Brain lysates after 0.5µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1,277(9):6806-12, PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184703 staining (0.5µg/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Yamaguchi, Okui, Yamada et al.: "Spinesin/TMPRSS5, a novel transmembrane serine protease, cloned from human spinal cord." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 6806-12, 2002 (PubMed).