Src Family Associated Phosphoprotein 2 (SKAP2) antibody

Antigen: Src Family Associated Phosphoprotein 2 (SKAP2)

Synonyms: PRAP, RA70, SAPS, SCAP2, SKAP55R, MGC10411, MGC33304, SKAP-HOM, SKAP2, prap, ra70, saps, scap2, skap55r, skap-hom, MGC128629

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184706

Additional Information

Alternative name: SCAP2 / PRAP

Gene ID: 8935

Immunogen: C-GLVPKAYIMEMYDI

Format: Affinity-purified

Description: All names and symbols: SCAP2, PRAP, src family associated phosphoprotein 2, MGC10411, MGC33304, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, src family associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adaptor protein. Official Symbol: SKAP2. RefSeq number(s): NP_003921.2. Gene ontology terms: signal transduction, SH3/SH2 adaptor protein, protein complex assembly

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). Recommended concentration: 0.5-2µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184706 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Takahashi, Yamashita, Nagano et al.: "Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 42225-33, 2003 (PubMed).

Park, Avraham, Avraham: "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33315-22, 2004 (PubMed).