Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) antibody

Antigen: Dickkopf 2 Homolog (Xenopus Laevis) (DKK2)

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184708

Additional Information

Alternative name: DKK2

Gene ID: 27123

Immunogen: ATYSSKARLHVCQKI

Description: All names and symbols: DKK2, dickkopf homolog 2 (Xenopus laevis), DKK-2, Dickkopf gene 2, dickkopf (Xenopus laevis) homolog 2,. Official Symbol: DKK2. RefSeq number(s): NP_055236. Gene ontology terms: extracellular space

Specificity: From sequence analysis it is possible that this product will crossreact with DKK4, unfortunately this has not yet been specifically tested. Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 8,000. Western Blot: Approx 28kDa band observed in Human Heart lysate (calculated MW of 28.5kDa according to NP_055236). Recommended concentration: 1-3µg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Wnt

Restrictions: For Research Use only

Images

ABIN184708 (2µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).