RP6-213H19.1 antibody

Antigen: RP6-213H19.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184713

Additional Information

Gene ID: 51765

Immunogen: KLIEKFQKCSADESP

Format: Affinity-purified

Description: All names and symbols: serine/threonine protein kinase MST4, RP6-213H19.1, MASK, MST4, Mst3 and SOK1-related kinase, STE20-like kinase MST4, mammalian Ste20-like protein kinase 4, mammalian sterile 20-like 4, serine/threonine protein kinase MASK. Official Symbol: RP6-213H19.1. RefSeq number(s): NP_057626.2, NP_001035918.1, NP_001035917.1

Specificity: This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1). Human, Mouse, Rat, Dog 416 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50kDa band observed in lysates of cell lines HEK293, HepG2 and Jurkat (calculated MW of 46.5kDa according to NP_057626.2). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184713 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Dan, Ong, Watanabe et al.: "Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 5929-39, 2002 (PubMed).