Kruppel-Like Factor 3 (Basic) (KLF3) antibody

Antigen: Kruppel-Like Factor 3 (Basic) (KLF3)

Synonyms: Bklf, Tef-2, AL024007, MGC151315, 9930027G08Rik, BKLF, MGC48279, klf12, KLF3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184727

Additional Information

Alternative name: KLF3 / BKLF

Gene ID: 51274

Immunogen: LMFDPVPVKQEAMD-C

Format: Affinity-purified

Description: All names and symbols: KLF3, BKLF, Kruppel-like factor 3 (basic), basic kruppel like factor. Official Symbol: KLF3. RefSeq number(s): NP_057615. Gene ontology terms: transcription factor, developmental processes, repression of transcription from Pol II promoter

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 40kDa band observed in Human Heart lysates (predicted MW of 40kDa according to NP_057615). Recommended for use at 1-3µg/ml. Please note that the 40kDa Band is sometimes accompanied by a 80-90kDa band of unknown identity.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184727 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Turner, Crossley: "Basic Kr" in: The international journal of biochemistry & cell biology, Vol. 31, Issue 10, pp. 1169-74, 2000 (PubMed).